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Category:Virtual reality Category:Panorama software Category:VRML Category:C Sharp softwareB2-N-oxide-activated induction of nitric oxide synthase isoform in macrophages: implication of intracellular glutathione. B2-N-oxides (BNOs) are formed when the halogen atoms of the compounds bromide and iodide are replaced by non-halogenated groups. All BNOs have anti-inflammatory effects and are known to be active at the level of immunologic and non-immunologic inflammation. Nitric oxide (NO), a potent inflammatory mediator of macrophages, is produced through inducible NO synthase (iNOS) upon lipopolysaccharide (LPS) stimulation. We studied the induction of NO production in murine macrophages (RAW 264.7) by BNOs. BNOs were found to induce NO in LPS-untreated cells by a NOx-GS-independent pathway and by NOx-GS-dependent mechanism in LPS-stimulated cells. The increase in NO production by BNOs was dose dependent, but the induction was not observed at low concentrations of BNOs. BNOs stimulated NO production in LPS-untreated RAW 264.7 cells by activation of protein kinase C (PKC) with low concentrations (1-10 microM). The NO production by BNOs was also correlated with increased activity of iNOS enzyme. BNOs were found to induce iNOS protein expression and iNOS activity in LPS-stimulated cells. Moreover, NO production induced by BNOs was correlated with the reduction in the intracellular glutathione (GSH) level. Inhibition of PKC by chelerythrine abolished the effect of BNOs on the reduced GSH level, but did not affect the NO production. These results show that BNOs act as modulators of iNOS through iNOS induction and iNOS activity. Furthermore, we suggest that iNOS activity is associated with the reduction in GSH level.In most engines, a pressure vessel made of cast steel has one or more openings through which fuel enters the combustion chamber and is burned. These are typically covered with rubber or other elastomeric seals in the combustion chamber and are often times compressed against the wall of the chamber as the engine is running. This causes friction and wear, especially

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